Gram negative shuttle BAC vector for heterologous expression of metagenomic libraries
نویسندگان
چکیده
منابع مشابه
Gram negative shuttle BAC vector for heterologous expression of metagenomic libraries.
Bacterial artificial chromosome (BAC) vectors enable stable cloning of large DNA fragments from single genomes or microbial assemblages. A novel shuttle BAC vector was constructed that permits replication of BAC clones in diverse Gram-negative species. The "Gram-negative shuttle BAC" vector (pGNS-BAC) uses the F replicon for stable single-copy replication in E. coli and the broad-host-range RK2...
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ژورنال
عنوان ژورنال: Gene
سال: 2011
ISSN: 0378-1119
DOI: 10.1016/j.gene.2010.11.004